ITGAL antibody

Catalog No. ABIN118562

The Mouse Monoclonal anti-ITGAL antibody has been validated for FACS, IP and IHC (fro). It is suitable to detect ITGAL in samples from Human.

Quick Overview for ITGAL antibody (ABIN118562)

Target: ITGAL (Integrin alpha-L (ITGAL))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ITGAL antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 38

Product Details anti-ITGAL Antibody

Specificity: This antibody recognises the integrin alpha-L subunit, a 180 kDa glycoprotein expressed by all human leucocytes. The molecule is involved in cell-cell adhesion processes and binds to ICAM-1, 2 and 3.

Purification: Affinity Chromatography on Protein G.

Isotype: IgG2a

Application Details

Application Notes: Flow cytometry: Use 5 μL of 1/50-1/100 diluted antibody to label 10^6 cells in 100 μL. Immunoprecipitation. Immunohistochemistry on frozen sections. Recommended positive control: Human Tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for ITGAL

Target: ITGAL (Integrin alpha-L (ITGAL))

Alternative Name: CD11a / ITGAL

Background: CD11a is a member of the integrin family of cell adhesion molecules. It is a glycoprotein expressed in combination with the CD18 beta chain. The complex is a member of the beta 2 integrin family. These molecules function in cell adhesion and specifically bind to CD54, ICAM2, ICAM3. CD11a is expressed on thymocytes, T and B lymphocytes, granulocytes, monocytes, and macrophages.Synonyms: CD11 antigen-like family member A, Integrin alpha-L, LFA-1, LFA1, Leukocyte adhesion glycoprotein LFA-1 alpha chain, Leukocyte function-associated molecule 1 alpha chain

Gene ID: 3683

NCBI Accession: NP_002200

UniProt: P20701

Pathways: Activated T Cell Proliferation, Integrin Complex